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Genome Sequencing of the Bornean Rock Frog

Submission Number: 50
Submission ID: 76
Submission UUID: cd575f43-d878-4f14-9f3d-cd97f1ab443f
Submission URI: /form/project

Created: Wed, 07/22/2020 - 11:45
Completed: Wed, 07/22/2020 - 12:16
Changed: Mon, 09/09/2024 - 14:14

Remote IP address: 24.34.98.209
Submitted by: Lisa Mangiamele
Language: English

Is draft: No
Webform: Project
Genome Sequencing of the Bornean Rock Frog
Northeast
pnas parvus.png
big-data (4), genomics (537), bioinformatics (277)
Complete

Project Leader

Lisa Mangiamele
413-454-1961
413-585-7028

Project Personnel

yaniv kovalski
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Project Information

We are working to sequence the genome of the Bornean Rock Frog, Staurois parvus. This frog uses a novel communication signal called "foot flagging", where it waves its back feet to send messages to other frogs. We currently have 220+GB of PacBio Sequel II genome sequence from an adult male frog. To make this genome a usable, accessible tool this sequence needs to be assembled, polished and annotated. This tool will help us investigate the evolution of new behaviors and how hormones regulate the nervous system.

Students working on this project will have the opportunity to work in the exciting field of amphibian genomics. Amphibians are notorious for having large complex genomes that are difficult to sequence and assemble. Due to this, students will have a very in-depth experience troubleshooting many steps of the sequencing and assembly pipeline. To date there have only been 10 frog genomes published, so students will have experience in a emerging field.

Project Information Subsection

Students working on this project will have the opportunity to work in the exciting field of amphibian genomics. Amphibians are notorious for having large complex genomes that are difficult to sequence and assemble. Due to this, students will have a very in-depth experience troubleshooting many steps of the sequencing and assembly pipeline. To date there have only been 10 frog genomes published, so students will have experience in a emerging field.
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Can work with any level
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Smith College
44 College Lane
Northampton, Massachusetts. 01063
NE-MGHPCC
07/23/2020
No
Already behind3Start date is flexible
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Once assembled this genome will be published.
Students will learn:
-Genome assembly pipelines using multiple assemblers (WTDBG2, Canu)
-Genome polishing
-Genome size estimation
-Possibly genome annotation
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Amphibian genomics is a unique field due to the large size and complexity of the genome. This poses challenges other genomics projects may not face. The Cyberteam program will learn about assembling large genomes and troubleshooting in genomics.
The most computationally expensive assembler we plan to run will generate 10-15 TB of intermediary data and use ~1TB of RAM
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Final Report

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